5EMB
Crystal structure of the SNX27 PDZ domain bound to the C-terminal phosphorylated PTHR PDZ binding motif
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-10-01 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.70850 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 37.570, 48.330, 56.177 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.200 - 0.850 |
| R-factor | 0.12397 |
| Rwork | 0.124 |
| R-free | 0.13567 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4z8j |
| RMSD bond length | 0.022 |
| RMSD bond angle | 2.089 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.265 | 0.860 |
| High resolution limit [Å] | 0.850 | 0.850 |
| Rmerge | 2.060 | |
| Number of reflections | 90806 | |
| <I/σ(I)> | 25.9 | 2.8 |
| Completeness [%] | 98.7 | 100 |
| Redundancy | 14 | 13.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | 10% PEG1000, 10% PEG8000 |
