5EMB

Crystal structure of the SNX27 PDZ domain bound to the C-terminal phosphorylated PTHR PDZ binding motif

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2015-10-01
Detector	ADSC QUANTUM 210r
Wavelength(s)	0.70850
Spacegroup name	P 21 21 21
Unit cell lengths	37.570, 48.330, 56.177
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.200 - 0.850
R-factor	0.12397
Rwork	0.124
R-free	0.13567
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4z8j
RMSD bond length	0.022
RMSD bond angle	2.089
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.7.0029)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	31.265	0.860
High resolution limit [Å]	0.850	0.850
Rmerge		2.060
Number of reflections	90806
<I/σ(I)>	25.9	2.8
Completeness [%]	98.7	100
Redundancy	14	13.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	10% PEG1000, 10% PEG8000