5EM9
Crystal structure of the SNX27 PDZ domain bound to the phosphorylated C-terminal 5HT4(a)R PDZ binding motif
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-10-01 |
Detector | ADSC QUANTUM 210r |
Wavelength(s) | 0.95370 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 37.219, 48.753, 57.059 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.066 - 1.600 |
R-factor | 0.1701 |
Rwork | 0.169 |
R-free | 0.19770 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4z8j |
RMSD bond length | 0.011 |
RMSD bond angle | 1.306 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.200 | 1.630 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.117 | 1.970 |
Number of reflections | 14298 | |
<I/σ(I)> | 14.4 | 2 |
Completeness [%] | 100.0 | 100 |
Redundancy | 14.2 | 14.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 4.5 | 293 | 40% PEG 400, 20% PEG8000, 0.1 M acetate (pH 4.5) |