5ELX

S. cerevisiae Dbp5 bound to RNA and mant-ADP BeF3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2014-08-21
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97918
Spacegroup name	P 21 21 21
Unit cell lengths	42.082, 91.751, 104.521
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	52.260 - 1.810
R-factor	0.177
Rwork	0.175
R-free	0.20800
RMSD bond length	0.005
RMSD bond angle	1.056
Data reduction software	HKL-2000
Data scaling software	Aimless (0.1.27)
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	104.520	1.910
High resolution limit [Å]	1.810	1.810
Rmerge	0.166	0.886
Number of reflections	37530
<I/σ(I)>	10.1	1.9
Completeness [%]	99.6	100
Redundancy	4.8	4.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	291	Mixed 100 nL of the complex at a protein concentration of 16 mg/ml with 100 nL of reservoir solution containing 200 mM Mg(NO3)2 and 18 percent PEG 3350 and incubating 4 days over 100 uL of reservoir solution in a sealed chamber.