5ELH

Crystal structure of mouse Unkempt zinc fingers 1-3 (ZnF1-3), bound to RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-12-06
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	1.282700
Spacegroup name	P 2 21 21
Unit cell lengths	43.194, 56.559, 131.025
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.941 - 1.800
R-factor	0.1801
Rwork	0.178
R-free	0.21550
RMSD bond length	0.007
RMSD bond angle	1.027
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	AutoSol
Refinement software	PHENIX

Data quality characteristics

	Overall
Low resolution limit [Å]	131.000
High resolution limit [Å]	1.800
Number of reflections	30245
<I/σ(I)>	14.6
Completeness [%]	99.4
Redundancy	6.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M lithium sulfate, 0.1 M Tris pH 8.5, 25% PEG 3350