5EL4
Structure of T. thermophilus 70S ribosome complex with mRNA and tRNALys in the A-site with a U-U mismatch in the first position
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-02-09 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.00 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 208.900, 447.800, 617.500 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 151.531 - 3.150 |
R-factor | 0.1935 |
Rwork | 0.193 |
R-free | 0.25110 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4wq1 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.715 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 200.000 | 200.000 | 3.250 |
High resolution limit [Å] | 3.150 | 50.000 | 3.150 |
Rmerge | 0.314 | 0.080 | 3.887 |
Rmeas | 0.082 | 3.976 | |
Total number of observations | 109004697 | ||
Number of reflections | 992033 | 284 | 88207 |
<I/σ(I)> | 31.6 | 62.4 | 0.97 |
Completeness [%] | 100.0 | 90.2 | 100 |
Redundancy | 109.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | PEG 20000, PEG 550mme, KSCN, Tris-OAc |