5EJQ

Structure of Dictyostelium Discoideum Myosin VII MyTH4-FERM MF1 domain, mutant 2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-02-11
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97934
Spacegroup name	P 21 21 21
Unit cell lengths	53.320, 61.480, 172.620
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.010 - 2.190
R-factor	0.1868
Rwork	0.185
R-free	0.21710
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ejy
RMSD bond length	0.010
RMSD bond angle	1.050
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER (2.5.6)
Refinement software	BUSTER-TNT (2.10.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.360	2.320
High resolution limit [Å]	2.190	2.190
Number of reflections	29999
<I/σ(I)>	18.15	6.14
Completeness [%]	100.0	99.97
Redundancy	8.8	8.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	290	10% PEG 20K, 20% PEG 550 MME, 0.1M MOPS pH 7.5, 0.1M HEPES pH 7.5, 20 mM DL-Alanine, 20 mM DL-Glutamic acid, 20 mM Glycine, 20 mM DL-Lysine HCl, 20 mM DL-Serine