5EJN
Crystal structure of Juno, the mammalian egg receptor for sperm Izumo1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-05-14 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.984000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 38.100, 52.200, 87.500 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.829 - 2.703 |
| R-factor | 0.2294 |
| Rwork | 0.233 |
| R-free | 0.24820 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4lrh |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.650 |
| Data reduction software | XDS (20151015) |
| Data scaling software | XDS (20151015) |
| Phasing software | PHASER (2.6.0) |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.830 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.072 | 0.774 |
| Number of reflections | 9510 | |
| <I/σ(I)> | 13.7 | 1.9 |
| Completeness [%] | 99.3 | 98.9 |
| Redundancy | 4.7 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 25% PEG 4000, 0.1 M Tris-HCl pH 8.5, 0.2 M calcium chloride |
