5EJC

Crystal structural of the TSC1-TBC1D7 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2014-07-23
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.000
Spacegroup name	C 1 2 1
Unit cell lengths	183.395, 66.704, 98.370
Unit cell angles	90.00, 91.91, 90.00

Refinement procedure

Resolution	50.000 - 3.100
R-factor	0.2206
Rwork	0.220
R-free	0.23930
Structure solution method	SAD
RMSD bond length	0.005
RMSD bond angle	0.996
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	AutoSol
Refinement software	REFMAC (5.8.0073)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.190
High resolution limit [Å]	3.100	7.090	3.100
Rmerge	0.108	0.045	0.758
Rmeas	0.128	0.053	0.905
Rpim	0.067	0.028	0.489
Total number of observations	74730
Number of reflections	21259
<I/σ(I)>	7.9
Completeness [%]	99.7	99.7	98.6
Redundancy	3.5	3.5	3.1
CC(1/2)		0.996	0.726

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	200 mM NaCl, 20mM CaCl2 and 15% PEG-3350