5EIY
Bacterial cellulose synthase bound to a substrate analogue
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-10-15 |
Detector | ADSC QUANTUM 1 |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 68.475, 216.654, 221.335 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.920 - 2.950 |
R-factor | 0.2229 |
Rwork | 0.222 |
R-free | 0.24810 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4p00 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.905 |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 35.000 |
High resolution limit [Å] | 2.900 |
Rmerge | 0.280 |
Number of reflections | 133706 |
<I/σ(I)> | 5.2 |
Completeness [%] | 99.3 |
Redundancy | 10.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 300 | sodium acetate |