5EIB

Crystal structure of CPAP PN2-3 C-terminal loop-helix in complex with DARPin-tubulin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2015-01-09
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9792
Spacegroup name	P 1 21 1
Unit cell lengths	73.856, 91.116, 83.310
Unit cell angles	90.00, 96.97, 90.00

Refinement procedure

Resolution	41.347 - 2.100
R-factor	0.1797
Rwork	0.178
R-free	0.22190
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4drx
RMSD bond length	0.010
RMSD bond angle	1.261
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.140
High resolution limit [Å]	2.100	5.700	2.100
Rmerge	0.128	0.040	0.899
Total number of observations	291375
Number of reflections	63572
<I/σ(I)>	8.1
Completeness [%]	99.1	99.5	98.2
Redundancy	4.6	4.6	4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.8	282	potassium sodium tartrate, PEG 3000