5EI9
Human PRDM9 allele-A ZnF Domain with Associated Recombination Hotspot DNA Sequence I
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-07-30 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 44.329, 55.740, 76.038 |
| Unit cell angles | 89.85, 75.50, 86.58 |
Refinement procedure
| Resolution | 32.980 - 1.921 |
| R-factor | 0.2153 |
| Rwork | 0.214 |
| R-free | 0.23730 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Zn SAD determined structure |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.117 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.980 | 2.020 |
| High resolution limit [Å] | 1.920 | 1.920 |
| Rmerge | 0.160 | 0.948 |
| Number of reflections | 52634 | |
| <I/σ(I)> | 16.39 | 2.6 |
| Completeness [%] | 99.9 | 99.6 |
| Redundancy | 24.9 | 13.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 289 | 0.058 M Bistris Propane, 0.042 M Citric acid, 25% PEG3350 |
