5EHK
Crystal structure of tRNA dependent lantibiotic dehydratase MibB from Microbispora sp. 107891
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2013-05-21 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 85.137, 151.112, 205.744 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.239 - 2.708 |
| R-factor | 0.2068 |
| Rwork | 0.204 |
| R-free | 0.26440 |
| Structure solution method | MIR |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.360 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHARP |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.750 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.065 | 0.772 |
| Number of reflections | 72174 | |
| <I/σ(I)> | 26.6 | 2 |
| Completeness [%] | 99.0 | 99.8 |
| Redundancy | 5.9 | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | 100 mM HEPES pH 7.5, 20-24% ethanol; 100 mM MOPS pH 7.9, 20-24% ethanol |
