5EHH
Structure of human DPP3 in complex with endomorphin-2.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-04-20 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.972 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 120.035, 105.457, 64.719 |
| Unit cell angles | 90.00, 93.49, 90.00 |
Refinement procedure
| Resolution | 49.090 - 2.380 |
| R-factor | 0.1959 |
| Rwork | 0.194 |
| R-free | 0.23700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3t6b |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.570 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHENIX (1.9_1692) |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.090 | 2.463 |
| High resolution limit [Å] | 2.380 | 2.380 |
| Rmerge | 0.091 | 0.632 |
| Number of reflections | 32020 | |
| <I/σ(I)> | 10.61 | 1.78 |
| Completeness [%] | 98.9 | 95.37 |
| Redundancy | 3.4 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.2 | 293.15 | 0.056 M Sodium phosphate monobasic monohydrate, 1.344 M Potassium phosphate dibasic, pH-8.2. |
