5EGI
Structure of a Trimeric Intracellular Cation channel from C. elegans with bound Ca2+
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-1A |
| Synchrotron site | Photon Factory |
| Beamline | BL-1A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-12-15 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 102.050, 102.050, 279.990 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 3.300 |
| Rwork | 0.263 |
| R-free | 0.27220 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.430 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | AutoSol |
| Refinement software | CNS (1.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.480 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmerge | 0.804 | |
| Number of reflections | 23166 | |
| <I/σ(I)> | 11.1 | 2.8 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 10.5 | 10.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 288 | 20-24% PEG400, 10% glycerol, 50 mM ADA buffer (pH6.5) |
