5EGB

Human PRDM9 allele-A ZnF Domain with Associated Recombination Hotspot DNA Sequence II

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	CCD
Collection date	2015-05-05
Detector	RIGAKU SATURN 944+
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	34.575, 79.221, 68.415
Unit cell angles	90.00, 95.42, 90.00

Refinement procedure

Resolution	29.639 - 1.977
R-factor	0.1939
Rwork	0.193
R-free	0.21960
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Zn SAD determined structure
RMSD bond length	0.009
RMSD bond angle	1.092
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.640	2.040
High resolution limit [Å]	1.970	1.970
Rmerge	0.071	0.712
Number of reflections	22386
<I/σ(I)>	25.3	1.9
Completeness [%]	86.1	37.6
Redundancy	7.7	4.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	289	0.055M Bis-Tris propane, 0.045M Citric Acid, 30% PEG3350