5EG9
The cap binding site of influenza virus protein PB2 as a drug target
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-07-15 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 37.133, 109.010, 39.655 |
| Unit cell angles | 90.00, 90.54, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.300 |
| R-factor | 0.1945 |
| Rwork | 0.191 |
| R-free | 0.25800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5eg8 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.638 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.340 |
| High resolution limit [Å] | 2.300 | 6.240 | 2.300 |
| Rmerge | 0.071 | 0.048 | 0.194 |
| Rmeas | 0.097 | 0.066 | 0.267 |
| Rpim | 0.066 | 0.044 | 0.183 |
| Total number of observations | 21876 | ||
| Number of reflections | 11663 | ||
| <I/σ(I)> | 13.1 | ||
| Completeness [%] | 87.7 | 82.5 | 92.6 |
| Redundancy | 1.8 | 1.9 | 1.7 |
| CC(1/2) | 0.986 | 0.909 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 293 | 10% PEG 3350 |
