5EG6

CSL-RITA complex bound to DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	200
Detector technology	CCD
Collection date	2013-07-23
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.978
Spacegroup name	P 21 2 21
Unit cell lengths	76.788, 96.410, 96.711
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.827 - 2.094
R-factor	0.1999
Rwork	0.198
R-free	0.23810
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.229
Data scaling software	HKL-2000
Phasing software	PHASER (2.5.3)
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.140
High resolution limit [Å]	2.094	5.700	2.094
Rmerge	0.074	0.046	0.536
Total number of observations	303810
Number of reflections	42943
<I/σ(I)>	7.6
Completeness [%]	99.8	99	98.6
Redundancy	7.1	6.5	5.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH		277	0.1M Bis-Tris pH5.5, 0.2M Ammonium Acetate, 10% 1,4-butanediol, and 16% polyethylene glycol 3350