5EG0
HOXB13-MEIS1 heterodimer bound to DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-11-06 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.972390 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 41.726, 56.332, 115.152 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.946 - 3.101 |
| R-factor | 0.2876 |
| Rwork | 0.285 |
| R-free | 0.32240 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4xrm |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.827 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 3.130 |
| High resolution limit [Å] | 2.970 | 2.970 |
| Rmerge | 0.280 | 2.770 |
| Number of reflections | 6006 | |
| <I/σ(I)> | 3.7 | 0.4 |
| Completeness [%] | 99.0 | 95.2 |
| Redundancy | 4.9 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | PEG 5000, potassium chloride, magnesium chloride, pentanol |
