5EFY
Apo-form of SCO3201
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.2 |
Synchrotron site | BESSY |
Beamline | 14.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-12-11 |
Detector | RAYONIX MX-225 |
Wavelength(s) | 0.918 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 51.790, 98.426, 89.611 |
Unit cell angles | 90.00, 100.78, 90.00 |
Refinement procedure
Resolution | 88.030 - 2.700 |
R-factor | 0.26037 |
Rwork | 0.258 |
R-free | 0.29733 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4cgr |
RMSD bond length | 0.012 |
RMSD bond angle | 1.443 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 94.600 | 2.850 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.500 | |
Number of reflections | 23495 | |
<I/σ(I)> | 7.7 | 1.44 |
Completeness [%] | 96.6 | |
Redundancy | 3 | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 295 | 12 % Ethanol; 0.1 M Tris/HCl; pH 5 |