5EFW

Crystal structure of LOV2-Zdk1 - the complex of oat LOV2 and the affibody protein Zdark1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-06-16
Detector	PSI PILATUS 6M
Wavelength(s)	0.9785
Spacegroup name	P 41 21 2
Unit cell lengths	54.700, 54.700, 188.030
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.280 - 2.100
R-factor	0.2045
Rwork	0.202
R-free	0.25660
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2v1a 1lp1
RMSD bond length	0.008
RMSD bond angle	1.064
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8_1069))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000		2.200
High resolution limit [Å]	2.100	10.000	2.100
Rmerge	0.104	0.070	0.528
Rmeas	0.115	0.077	0.577
Total number of observations	100397
Number of reflections	17450	204	2220
<I/σ(I)>	10.3	20.6	3.26
Completeness [%]	99.4	94.9	100
Redundancy	5.8		6.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	3.5	293.15	2 M ammonium sulfate, 0.1 M sodium citrate pH 3.5