5EFG
Crystal structure of Danio rerio histone deacetylase 6 catalytic domain 2 in complex with acetate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 4.2.2 |
Synchrotron site | ALS |
Beamline | 4.2.2 |
Temperature [K] | 100 |
Detector technology | CMOS |
Collection date | 2015-10-01 |
Detector | RDI CMOS_8M |
Wavelength(s) | 1.00003 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 75.123, 91.852, 96.206 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.766 - 2.250 |
R-factor | 0.2135 |
Rwork | 0.212 |
R-free | 0.23430 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5eek |
RMSD bond length | 0.003 |
RMSD bond angle | 0.847 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.3_1479) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.400 |
High resolution limit [Å] | 2.250 | 2.250 |
Rmerge | 0.130 | 0.356 |
Number of reflections | 32201 | |
<I/σ(I)> | 14.5 | 2.8 |
Completeness [%] | 99.3 | 93.9 |
Redundancy | 6.3 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.2 M sodium acetate, 30% PEG8000 |