5EF6
Structure of HOXB13 complex with methylated DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-05-26 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.97242 |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 77.969, 55.557, 102.010 |
| Unit cell angles | 90.00, 102.24, 90.00 |
Refinement procedure
| Resolution | 38.169 - 3.000 |
| R-factor | 0.2923 |
| Rwork | 0.291 |
| R-free | 0.32000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5edn |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.994 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 55.160 | 55.160 | 3.160 |
| High resolution limit [Å] | 2.980 | 8.940 | 2.980 |
| Rmerge | 0.202 | 0.041 | 0.864 |
| Rmeas | 0.241 | 0.050 | 1.026 |
| Rpim | 0.130 | 0.028 | 0.549 |
| Total number of observations | 57890 | 2054 | 9303 |
| Number of reflections | 17019 | ||
| <I/σ(I)> | 5 | 13.3 | 1.4 |
| Completeness [%] | 97.9 | 96.3 | 96.4 |
| Redundancy | 3.3 | 3 | 3.4 |
| CC(1/2) | 0.988 | 0.999 | 0.781 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | PEG 3350, potassium chloride, magnesium chloride, 2-methyl-1-propanol, Tris |
