5EEO
soybean lipoxygenase(L1)-T756R
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-05-10 |
| Detector | RIGAKU SATURN A200 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 70.708, 62.361, 92.351 |
| Unit cell angles | 90.00, 103.25, 90.00 |
Refinement procedure
| Resolution | 38.700 - 2.100 |
| R-factor | 0.1892 |
| Rwork | 0.186 |
| R-free | 0.25440 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1f8n |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.137 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.700 | 2.140 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.108 | 0.448 |
| Number of reflections | 45398 | |
| <I/σ(I)> | 28.4 | 7.2 |
| Completeness [%] | 98.8 | 100 |
| Redundancy | 5.8 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2M Lithium chloride, 20%(w/v) Polyethylene glycol 3,350 |
