5EEM
Crystal structure of Danio rerio histone deacetylase 6 catalytic domain 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-08-15 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 1 |
| Unit cell lengths | 48.485, 55.400, 74.183 |
| Unit cell angles | 73.58, 89.84, 82.64 |
Refinement procedure
| Resolution | 15.910 - 2.000 |
| R-factor | 0.226 |
| Rwork | 0.224 |
| R-free | 0.27270 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5eek |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.878 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.3_1479) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.150 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.162 | 0.501 |
| Number of reflections | 47957 | |
| <I/σ(I)> | 6.4 | 1.8 |
| Completeness [%] | 97.0 | 96.6 |
| Redundancy | 1.9 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.1 M sodium acetate, 0.1 M sodium formate, 20% PEG3350 |
