5EEF
Crystal structure of Danio rerio histone deacetylase 6 catalytic domain 1 in complex with trichostatin A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL14-1 |
| Synchrotron site | SSRL |
| Beamline | BL14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-07-23 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 1.28184 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.876, 123.717, 55.186 |
| Unit cell angles | 90.00, 113.85, 90.00 |
Refinement procedure
| Resolution | 45.040 - 2.151 |
| R-factor | 0.2128 |
| Rwork | 0.211 |
| R-free | 0.25480 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5eek |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.788 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.3_1479) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.040 | 2.600 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.178 | 0.701 |
| Number of reflections | 34861 | |
| <I/σ(I)> | 10.6 | 2 |
| Completeness [%] | 99.0 | 95.9 |
| Redundancy | 3.1 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | 0.2 M ammonium sulfate, 0.1 M MES, pH 6.5, 25% PEG5000 MME |
