5EDP
EGFR kinase (T790M/L858R) apo
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08B1-1 |
| Synchrotron site | CLSI |
| Beamline | 08B1-1 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2012-02-11 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 1.0332 |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 148.847, 148.847, 148.847 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.070 - 2.900 |
| R-factor | 0.2016 |
| Rwork | 0.200 |
| R-free | 0.24410 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.070 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.5) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 47.100 |
| High resolution limit [Å] | 2.900 |
| Number of reflections | 12322 |
| <I/σ(I)> | 23.8 |
| Completeness [%] | 100.0 |
| Redundancy | 11.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 292 | PEG 10K, ethylene glycol |
