5EDN
Structure of HOXB13-DNA(TCG) complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-11-06 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.97239 |
| Spacegroup name | P 2 2 21 |
| Unit cell lengths | 52.618, 52.522, 389.331 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.290 - 3.200 |
| R-factor | 0.21934 |
| Rwork | 0.216 |
| R-free | 0.28130 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4xrm |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.569 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0123) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.290 | 3.370 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.097 | 0.653 |
| Number of reflections | 17652 | |
| <I/σ(I)> | 6.7 | 1.9 |
| Completeness [%] | 93.7 | 81.8 |
| Redundancy | 4.4 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | PEG 3350, potassium chloride, magnesium chloride, PEG 400, Tris |
