5EDK
Crystal structure of prothrombin deletion mutant residues 146-167 ( Form II ).
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2014-06-27 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 84.192, 84.192, 346.427 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 86.610 - 3.214 |
| R-factor | 0.29274 |
| Rwork | 0.291 |
| R-free | 0.32266 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | pdb code 4O03 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.211 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 86.610 | 3.260 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.155 | 0.441 |
| Number of reflections | 20053 | |
| <I/σ(I)> | 7 | 2 |
| Completeness [%] | 94.5 | 94 |
| Redundancy | 4.4 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9.1 | 293 | 0.2 M di-Na hydrogen phosphate and 20% PEG 3350 |
