5ECP
Crystal Structure of FIN219-FIP1 complex with JA, MET and ATP
Experimental procedure
| Experimental method | LAUE |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13C1 |
| Synchrotron site | NSRRC |
| Beamline | BL13C1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-11-08 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 0.97620-0.97622 |
| Spacegroup name | P 1 |
| Unit cell lengths | 53.708, 53.701, 192.029 |
| Unit cell angles | 89.96, 90.16, 113.24 |
Refinement procedure
| Resolution | 23.347 - 2.250 |
| R-factor | 0.185207158756 |
| Rwork | 0.185 |
| R-free | 0.20767 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4epl |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.639 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (2.5.6) |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.330 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.131 | 0.406 |
| Number of reflections | 89193 | |
| <I/σ(I)> | 4.6 | 1.992 |
| Completeness [%] | 93.6 | 96.7 |
| Redundancy | 1.9 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 297 | 0.2 M sodium acetate trihydrate, 0.1 M Tris-HCl, 30%(w/v) PEG 3000 |
