5ECJ

Crystal structure of monobody Mb(S4) bound to Prdm14 in complex with Mtgr1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2014-07-13
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97918
Spacegroup name	P 43 21 2
Unit cell lengths	106.812, 106.812, 180.830
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.764 - 3.050
R-factor	0.1939
Rwork	0.189
R-free	0.24960
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	1.074
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX (1.9_1692)
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.764	3.160
High resolution limit [Å]	3.050	3.050
Number of reflections	20631
<I/σ(I)>	30
Completeness [%]	99.9	100
Redundancy	20

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	17% PEG3350, 8% Tacsimate