5EBZ
Crystal structure of human IKK1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-11-04 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97927 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 174.510, 186.940, 275.830 |
| Unit cell angles | 90.00, 98.84, 90.00 |
Refinement procedure
| Resolution | 29.940 - 4.500 |
| R-factor | 0.238 |
| Rwork | 0.238 |
| R-free | 0.27600 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.800 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | CNS (1.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.000 | 4.580 |
| High resolution limit [Å] | 4.500 | 4.500 |
| Rmerge | 0.100 | 0.810 |
| Number of reflections | 99808 | |
| <I/σ(I)> | 5.2 | 1.5 |
| Completeness [%] | 95.5 | 88 |
| Redundancy | 7 | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 277 | PEG, Dextran Sulfate |
