5EAX

Crystal structure of Dna2 in complex with an ssDNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-11-01
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.987
Spacegroup name	P 21 21 21
Unit cell lengths	120.234, 149.211, 172.900
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.990 - 3.050
R-factor	0.2234
Rwork	0.223
R-free	0.25620
RMSD bond length	0.009
RMSD bond angle	1.430
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.7.0032)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	3.110
High resolution limit [Å]	3.000	6.440	3.000
Rmerge	0.137	0.040
Rmeas	0.150	0.044
Rpim	0.066	0.018	0.571
Total number of observations	379814
Number of reflections	62189
<I/σ(I)>	6.9
Completeness [%]	99.0	96.4	99.2
Redundancy	6.1	6	6
CC(1/2)		0.999	0.701

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	IPA, CaCl2, MES pH6.5