5EAP
Crystal structure of human WDR5 in complex with compound 9e
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-01-25 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 0.97931 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 97.162, 48.232, 63.448 |
| Unit cell angles | 90.00, 108.01, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.730 |
| R-factor | 0.2057 |
| Rwork | 0.204 |
| R-free | 0.24090 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ur4 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.421 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.760 |
| High resolution limit [Å] | 1.730 | 4.690 | 1.730 |
| Rmerge | 0.079 | 0.059 | 0.452 |
| Total number of observations | 120247 | ||
| Number of reflections | 29229 | ||
| <I/σ(I)> | 9.4 | ||
| Completeness [%] | 99.2 | 99.7 | 92.5 |
| Redundancy | 4.1 | 4 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 28% PEG 2K MME, 0.1 M BisTris pH6.5 |
