5E9W
Crystal structure of mRNA cap guanine-N7 methyltransferase obtained by limited proteolysis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-05-14 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 77.458, 99.157, 167.614 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.578 - 2.283 |
| R-factor | 0.2219 |
| Rwork | 0.220 |
| R-free | 0.25500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3bgv |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.342 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | PHENIX (dev_1702) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.580 | 2.410 |
| High resolution limit [Å] | 2.280 | 2.280 |
| Rmerge | 0.080 | 0.270 |
| Number of reflections | 57298 | |
| <I/σ(I)> | 10.3 | 3.9 |
| Completeness [%] | 97.0 | 97.6 |
| Redundancy | 2.8 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | MES pH 6.5 and 25% PEG 4000, 1/5000 thermolysin |
