5E9W

Crystal structure of mRNA cap guanine-N7 methyltransferase obtained by limited proteolysis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-05-14
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	77.458, 99.157, 167.614
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.578 - 2.283
R-factor	0.2219
Rwork	0.220
R-free	0.25500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3bgv
RMSD bond length	0.008
RMSD bond angle	1.342
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	PHENIX (dev_1702)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.580	2.410
High resolution limit [Å]	2.280	2.280
Rmerge	0.080	0.270
Number of reflections	57298
<I/σ(I)>	10.3	3.9
Completeness [%]	97.0	97.6
Redundancy	2.8	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	MES pH 6.5 and 25% PEG 4000, 1/5000 thermolysin