5E9T

Crystal structure of GtfA/B complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	80
Detector technology	CCD
Collection date	2014-08-16
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9792
Spacegroup name	I 2 2 2
Unit cell lengths	155.930, 196.410, 220.860
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	122.123 - 2.920
R-factor	0.2199
Rwork	0.217
R-free	0.27500
Structure solution method	SAD
Starting model (for MR)	4pqg
RMSD bond length	0.010
RMSD bond angle	1.346
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	122.123	122.100	2.670
High resolution limit [Å]	2.920	11.340	2.600
Rmerge	0.239	0.055
Rmeas	0.312	0.056
Total number of observations	5781655
Number of reflections	73613	1363	7993
<I/σ(I)>	23.13	77.62	0.2
Completeness [%]	99.9	98.4	99.8
Redundancy	58.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	295	PEG4000, magnesium chloride, Tris