5E9S
Crystal structure of substrate-bound glutamate transporter homologue GltTk
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-10-03 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 117.520, 117.520, 310.590 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.358 - 2.800 |
| R-factor | 0.2136 |
| Rwork | 0.213 |
| R-free | 0.24250 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ky0 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.131 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10_2155)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.400 | 2.870 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.070 | |
| Number of reflections | 60568 | |
| <I/σ(I)> | 9.3 | 1.3 |
| Completeness [%] | 97.5 | 97 |
| Redundancy | 3 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 20% PEG 400, 3% Xylitol, 50mM MgCl2, 150mM NaCl, 100mM Glycine pH 9 |
