5E8L

Crystal structure of geranylgeranyl pyrophosphate synthase 11 from Arabidopsis thaliana

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	80
Detector technology	CCD
Collection date	2013-10-08
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.987
Spacegroup name	P 21 21 21
Unit cell lengths	63.111, 97.831, 109.814
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.145 - 2.807
R-factor	0.1905
Rwork	0.188
R-free	0.23650
RMSD bond length	0.012
RMSD bond angle	1.709
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.800
Number of reflections	16227
<I/σ(I)>	13.7
Completeness [%]	95.6
Redundancy	5.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	293	0.4 M Magnesium formate dihydrate, 0.1 M sodium acetate trihydrate, pH 4.6