5E8F
Structure of Fully modified geranylgeranylated PDE6C Peptide in complex with PDE6D
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-09-12 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.00001 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 77.710, 81.430, 118.530 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.630 - 2.100 |
| R-factor | 0.2012 |
| Rwork | 0.198 |
| R-free | 0.25610 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3t5g |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.973 |
| Data reduction software | XDS |
| Data scaling software | XSCALE (5.7.0032) |
| Phasing software | MOLREP (3.15) |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.630 | 2.200 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 22232 | |
| <I/σ(I)> | 23.46 | 10.24 |
| Completeness [%] | 99.6 | 100 |
| Redundancy | 5.29 | 5.35 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M HEPES (pH 7.5), 0.2 M Li2SO4, 25 % PEG4000 and 0.1 M NaOAc |
