5E7B

Structure of a nanobody (vHH) from camel against phage Tuc2009 RBP (BppL, ORF53)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-06-06
Detector	PSI PILATUS 6M
Wavelength(s)	0.86
Spacegroup name	P 65 2 2
Unit cell lengths	52.080, 52.080, 162.690
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	14.970 - 1.100
R-factor	0.1595
Rwork	0.158
R-free	0.18920
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.367
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.100	1.130
High resolution limit [Å]	1.100	1.100
Rmerge	0.068	1.550
Number of reflections	52014
<I/σ(I)>	18.4	1.5
Completeness [%]	96.6	91.7
Redundancy	19.5	19.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.4	293	mixing 100-300 nl of 12 mg.mL-1 protein (Na2HPO4, 10 mM; KH2PO4, 1.8 mM, pH7.4; NaCl, 137 mM; KCl, 2.7mM) with 100 nl precipitant solution in 25-30 % PEG4000, 0.2 M Imidazole (pH 6.0).