5E71

Crystal structure of the archaeal tRNA m2G/m22G10 methyltransferase (aTrm11) from Thermococcus kodakarensis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL38B1
Synchrotron site	SPring-8
Beamline	BL38B1
Temperature [K]	100
Detector technology	CCD
Collection date	2014-06-11
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	47.733, 53.520, 134.859
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.442 - 1.700
R-factor	0.2065
Rwork	0.205
R-free	0.23950
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	1.027
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1682)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.730
High resolution limit [Å]	1.700	4.610	1.700
Rmerge	0.051	0.027	0.594
Rmeas	0.055	0.029	0.641
Rpim	0.021	0.011	0.238
Total number of observations	275794
Number of reflections	38847
<I/σ(I)>	17
Completeness [%]	99.8	97.4	100
Redundancy	7.1	6.6	7.2
CC(1/2)		0.999	0.951

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	4.5M NaCl, 0.1M Tris-HCl