5E6S
Structures of leukocyte integrin aLB2: The aI domain, the headpiece, and the pocket for the internal ligand
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2012-04-07 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.03321 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 151.900, 118.877, 150.967 |
| Unit cell angles | 90.00, 118.52, 90.00 |
Refinement procedure
| Resolution | 49.450 - 2.150 |
| R-factor | 0.1958 |
| Rwork | 0.194 |
| R-free | 0.23400 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.800 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.150 |
| Number of reflections | 180557 |
| <I/σ(I)> | 5.2 |
| Completeness [%] | 70.5 |
| Redundancy | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 277 | 0.1 M Tris, pH 8, 0.1 M NaCl, and 8% polyethylene glycol (PEG) 20,000 |
