5E6Q

Importin alpha binding to XRCC1 NLS peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2013-06-01
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	78.668, 90.112, 100.699
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.407 - 2.305
R-factor	0.1753
Rwork	0.173
R-free	0.20820
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3uky
RMSD bond length	0.002
RMSD bond angle	0.680
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.300
Number of reflections	31129
<I/σ(I)>	9.6
Completeness [%]	96.6
Redundancy	5.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	1.5M Ammonium sulfate, 0.1M bis-tris propane