5E6C
Glucocorticoid receptor DNA binding domain - CCL2 NF-kB response element complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-07-02 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.00 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 39.083, 97.407, 103.852 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.523 - 2.200 |
R-factor | 0.237 |
Rwork | 0.233 |
R-free | 0.27060 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.178 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.700 | 2.280 |
High resolution limit [Å] | 2.200 | 2.200 |
Number of reflections | 20451 | |
<I/σ(I)> | 20.9 | 2.1 |
Completeness [%] | 98.3 | 87.8 |
Redundancy | 6.7 | 2.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.1 M sodium malonate, 6% glycerol, 5% PEG 3350 |