5E6A

Glucocorticoid receptor DNA binding domain - PLAU NF-kB response element complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2014-07-01
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	39.993, 95.497, 103.634
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.114 - 2.197
R-factor	0.2389
Rwork	0.236
R-free	0.26580
RMSD bond length	0.005
RMSD bond angle	0.842
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.230
High resolution limit [Å]	2.150	2.150
Number of reflections	20243
<I/σ(I)>	16.2	1.67
Completeness [%]	100.0	100
Redundancy	7.1	6.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	0.05 M sodium cacodylate, 0.05 M spermine, and 16% PEG 400