5E5V

Structure of amyloid forming peptide NFGAILS (residues 22-28) from Islet Amyloid Polypeptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	291
Detector technology	CCD
Collection date	2013-03-22
Detector	ADSC QUANTUM 315
Wavelength(s)	0.979
Spacegroup name	P 1
Unit cell lengths	8.661, 11.594, 21.552
Unit cell angles	86.39, 82.21, 76.35

Refinement procedure

Resolution	21.342 - 1.240
R-factor	0.1759
Rwork	0.173
R-free	0.20620
RMSD bond length	0.008
RMSD bond angle	1.024
Data reduction software	DENZO
Phasing software	PHASER
Refinement software	PHENIX (1.8.1_1168)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	1.340
High resolution limit [Å]	1.240	1.280
Rmerge	0.166	0.558
Number of reflections	2283
<I/σ(I)>	6.48	1.6
Completeness [%]	97.8	96.9
Redundancy	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	7 mg/ml in water and mixed with 10% ethanol and 1.5M NaCl