5E5O
I-SmaMI bound to uncleaved DNA target in the presence of Calcium ions
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-09-10 |
| Detector | RIGAKU SATURN 70 |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 61.327, 67.993, 97.743 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.000 - 2.360 |
| R-factor | 0.19209 |
| Rwork | 0.188 |
| R-free | 0.27353 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4lox |
| RMSD bond length | 0.017 |
| RMSD bond angle | 2.086 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0124) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.000 | 2.440 |
| High resolution limit [Å] | 2.360 | 2.360 |
| Rmerge | 0.122 | 0.809 |
| Number of reflections | 17265 | |
| <I/σ(I)> | 14.8 | 2 |
| Completeness [%] | 99.2 | 93.3 |
| Redundancy | 6.9 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | PEG MME550, HEPES |
