5E5M

Crystal structure of mouse CTLA-4 in complex with nanobody

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 31-ID
Synchrotron site	APS
Beamline	31-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2015-07-24
Detector	RAYONIX MX225HE
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	78.220, 111.572, 112.881
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.969 - 2.182
R-factor	0.195
Rwork	0.193
R-free	0.24670
RMSD bond length	0.008
RMSD bond angle	1.080
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.8_1069)

Data quality characteristics

	Overall
Low resolution limit [Å]	19.969
High resolution limit [Å]	2.182
Number of reflections	98782
<I/σ(I)>	7.4
Completeness [%]	99.7
Redundancy	4.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.2	293	Sodium/Potassium Phosphate