5E56
Crystal structure of mouse CTLA-4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-07-08 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 0.97931 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 47.538, 36.598, 65.149 |
| Unit cell angles | 90.00, 105.13, 90.00 |
Refinement procedure
| Resolution | 27.805 - 1.504 |
| R-factor | 0.1809 |
| Rwork | 0.180 |
| R-free | 0.20270 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dqt |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.064 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.8_1069) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 27.805 |
| High resolution limit [Å] | 1.504 |
| Number of reflections | 17192 |
| <I/σ(I)> | 12.8 |
| Completeness [%] | 99.1 |
| Redundancy | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 293 | Na/K Phosphate |
