5E55
Crystal structure of mouse CNTN6 FN1-FN3 domains
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-03-13 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.00 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 86.744, 90.847, 99.349 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.372 - 2.702 |
R-factor | 0.2019 |
Rwork | 0.200 |
R-free | 0.24080 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5e4s |
RMSD bond length | 0.004 |
RMSD bond angle | 0.917 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.9) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.800 |
High resolution limit [Å] | 2.700 | 5.810 | 2.700 |
Rmerge | 0.155 | 0.074 | 0.515 |
Total number of observations | 160904 | ||
Number of reflections | 21656 | ||
<I/σ(I)> | 8.4 | ||
Completeness [%] | 97.9 | 96 | 92.5 |
Redundancy | 7.4 | 8.1 | 4.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 300 mM Na-Malonate pH 7.0, 20% (w/v) PEG 3350, 50 mM Na-Cacodylate pH 6.5 |