5E4G

Crystal structure of human growth differentiation factor 11 (GDF-11)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-02-06
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.00
Spacegroup name	P 41 21 2
Unit cell lengths	32.270, 32.270, 210.602
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	22.818 - 1.500
R-factor	0.189
Rwork	0.188
R-free	0.20710
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	homology model
RMSD bond length	0.006
RMSD bond angle	1.058
Data reduction software	d*TREK
Data scaling software	d*TREK
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	22.820	22.820	1.550
High resolution limit [Å]	1.500	3.230	1.500
Rmerge	0.056	0.034	0.560
Total number of observations	56267	5676	5275
Number of reflections	17599
<I/σ(I)>	9.4	31.7	1.9
Completeness [%]	92.3	83.4	91.8
Redundancy	3.11	3.11	3.01

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	0.18 M magnesium acetate, 0.1 M HEPES, pH 7.5, 22.5% PEG3350, 0.01 M strontium chloride